Proceed with the following steps using optimized settings. 3. For more sample throughput, the system can Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 8. 5 Z’ factor 0. Bookings are free, but you will need to use iLab to book. Absorbance at a wavelength of 450 nm was measured using the SpectraMax M5e multi-mode microplate reader (Molecular Devices, San Jose, CA, USA) to quantify cytokine. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 0 17. Dual monochromators for assay flexibility. 0. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax® M5e Multi-Mode Microplate Reader (Molecular Devices) Assay setup and detection. Both instruments demonstrated good performance for the assay. variety of. The FlexStation 3 Reader combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. 1. spr SpectraMax iD3 SpectraTest ABS2 v1. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. M5e: W = 15. SpectraMax M5e: 118. 2222 Fifth Street. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. Microplate Readers include the M3, M4, exception is noted. Your lab might even have one already. NanoRacks reconfigured the SpectraMax M5e, one of Molecular Devices’ most reliable, durable, and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. 3. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. The donor and acceptor can be used to label a variety of biomolecules, for applications. KEY FEATURES Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. edu) to be added as a trained user in iLab. 1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Page 75. 90 0. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. M3-M5e_manual. The CellTiter-Glo® 2. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe SpectraMax M5e, one of Molecular Devices most reliable and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. 8. 316 grating motor driver faulty. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. 28 nM to 0. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. The SpectraMax® M5e Microplate Reader's unique mechanics allows efficient adaptation to the zero gravity in space and offers the most advanced features in one unit, making it the ideal candidate for handling research applications in a rigorous environment. 1. $. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. For more information please visit us at: SpectraMax®i3Multi-ModeMicroplateReader* SpectraMax®i3xMulti-ModeMicroplateReader*. HTRF测定在SpectraMax M5e读板机上的 表现通过使用两种不同的HTRF检测试剂 展示。G-蛋白偶联受体信号转导通过检测 两种主要通路，cAMP的调控和IP3介导的 胞内钙离子水平增加。利用cAMP dynamic 2试剂中Eu的穴状化合物标记的 单克隆抗-cAMP抗体和d2-标记的cAMP进 行检测。The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 40266 Email: drugdiscoverytech@lifetech. 1. Setup Guide On The Molecular Devices SpectraMax®SpectraMax L: 36. , contact Molecular. Detection modes include: • Endpoint, kinetic, spectral and well-scanning • Comprehensive data analysis • Robotics compatibility • Fluorescence Intensity (FI) • Time-Resolved Fluorescence (TRF) The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. SpectraMax Mini: 17. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. SpectraMax Absorbance, Fluorescence, Luminescence, and Multi-Mode Readers. 00 $ 2,999. 00We would like to show you a description here but the site won’t allow us. Enzyme titration and time course, cofactor and substrate Km,andIC50 for known inhibitors are demonstrated. 90 Read time (384 wells) 5:03 2:17 14:00 Table 3. Products : SpectraMax M5/M5e reader Fluorescence-based mitochondrial function analysis (PDF) Summary: Mitochondrial dysfunction is implicated in numerous disease states and is also a major mechanism of drug-induced toxicity. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. 1. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. Bioz Stars score: 86/100, based on 1 PubMed citations. com 10. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Multi-Mode Microplate Readers. Real-time fluorescence signals were recorded using CFX96 thermocycler (Bio-Rad). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 5 mP standard deviation at 1 nMF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. The drawing shows a standard microplate of 15 mm height covered with a lid. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. ISM Equipment Classification (Group 1, Class A) This equipment is. AquaMax® 4000. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. OD 450 nm was measured by SpectraMax M5e. 多功能酶标仪 FlexStation3 快速动力学操. #M5e) Methods Microplate reader compatibility test. 8 x 10-15. 313 reference gain check fail. 1. The optical systemsApplication note demonstrating that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Ferguson, Ph. In the M2, M5 and Plus 384, the light. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Jun 9th, 2023. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. 1. 2 x 10-14. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seFigure 2: Expression of mCherry with (improved BBa_K1141000) and without (BBa_K1141000) the translation enhancing 5’ untranslated region was measured (Ex: 587 nm, Em: 610 nm) over a period of 6 hours after induction with 0. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter. Note: In this user guide, all references to SpectraMax Multi-Mode. Paradigm: W = 21. Reads various kinds of 96-wells plates, and is equipped with shaking function. 1. Lift up the lid over the chamber to insert or remove a cuvette. Upgradeable modules are also available including western blot, cell imaging, and fast. 9000-0756. 6 x 10-15. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. No special upgrade is required for HTRF ® readout, as it is a monochromator-based instrument. E3300S/L) can be found on the New. The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. Choose from a three- (M3), four- (M4), orSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideGeneration of NADPH was monitored by fluorescence (Ex340/Em460 nm) every minute for 15 minutes on a SpectraMax M5e plate reader (Molecular Devices at 25°C). These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Dual monochromators for assay flexibility With. View the Molecular Devices website. 92 0. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. . Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. SpectraMax® L (software version 5. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. Once the M5e Reader is operational, space-based researchers will be able to conduct analysis on-orbit. The SpectraMax M5e reader offers the additional benefit of being certified . This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. 1. The KeySpan USA-19HS is often required with new Windows 7. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 8 x 10-15. 00. 1. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. View full details Original price $5,513. 5. 485. The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. 5 mP standard deviation at 1 nM. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The user manual is an indispensable documentation for of any product. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument. 1, left panel) and individual wells (Fig. S. 40266 Email: drugdiscoverytech@lifetech. 1. 3: The cuvette chamber. 1, right panel), for rapid, real-time high throughput screening of mitochondrial (dys) function. Supplier: Molecular Devices. 00 USD. the performance of the Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader with an integrated 8-channel or 16-channel pipettor into one compact benchtop reader. 8 x 10-15. Find regional support. SpectraMax M5. SpectraMax M5e. SpectraMax M5e: 118. 5. The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Under these assay conditions, up to 75. The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® Assays. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Conclusions. Oxygen consumption is one of the most informative and direct measures of mitochondrial function. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. 标准功. HTRF is compatible with a large range of available microplate readers. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode. Luciferase Detection Instrumentation Guide. SpectraMax M5e: 118. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 5, Z’ = 0. Recertification is recommended every two years. Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and other niche laboratory equipment lines. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. for Cisbio Bioassays HTRF® technology. SpectraMax M3, M4, M5, and M5e Multi-Mode Microplate Readers 0112-0115 F 9Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. SpectraMax M5e Reader only Certified to Cisbio Bioassays HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. Two cynomolgus monkeys (1 male and 1 female) weighing 2–4 kg and 3–5 years old were purchased from Suzhou. The mirror set. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 8. Rating: 5. book Page 1 Wednesday, July 7, 2010 2:09 PM. When a feature or capability applies to only certain readers, this exception is noted. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 FlexStation® 3 Multi-Mode Microplate Reader. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well7. GE AKTA™ Crossflow. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . 0. 1. The optical systemsSpectraMax L: 36. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SpectraMax Mini: 17. HTRF is a versatile technology developed by Cisbio Bioassays for detecting. Molecular. 0 Application Area:. SpectraMax M5eは、2011年に国際ステーションで使用されるプレートリーダーとして初めて採用されました。 SpectraMAx Mシリーズプレートリーダーは発売以来、「壊れにくい装置」として認知されており、今なお採用され続けている実績のある装置です。Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader Why does "Path?" appears in all wells when PathCheck is selected? Cause: Water Constants. All associated StakMax® stackers. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. FlexStation. M5/M5e Multi-Mode Microplate Reader SpectraMax. Download Operation & user’s manual of Molecular Devices SpectraMax M3 Laboratory Equipment for Free or View it Online on All-Guides. The resulting purple crystals were dissolved in solubilization buffer and analyzed by spectrophotometry at 490 nm, using a reference of 650 nm in a microplate reader (SpectraMax M5e, MD, USA. G-protein coupled receptors signal through two major pathways, regulation of cAMP and increase in intracellular calcium levels mediated by IP3. D. It was also read on the monochromator-based SpectraMax® M5e Multi-Mode Microplate Reader using optimized wavelengths. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. Dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The schematic of the UED setup is shown as Fig. Choose cables that meet the requirement described in this appendix. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 40266 Email: drugdiscoverytech@lifetech. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. 6 x 10-15. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Venue Address. 1. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. 00. Dual monochromators for assay flexibility. 2 x 10-14. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The dual monochromator optics allow. WARNING! The instrument weighs approximately 36 pounds. The Spectr. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 1. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 6 x 10-15. Also refer to this Multimode Reader Comparison Chart. book Page 1 Wednesday, July 7, 2010 2:09 PM. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. . The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The measurements were performed in triplicate. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. 5. 1. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s. Offer Ends In: 18 Days 19: 38: 21. The following day, calcium content was quantified by the QuantiChrom™ Calcium Kit (BioAssay Systems) by reding absorbance at 612 nm using the Spectramax M5e reader (Molecular Devices). 4, Z’ = 0. SpectraMax M5e: 118. 2 x 10-14. The optical systemsThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 1. 4, Z’ = 0. 00 USD. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. SoftMax Pro 软件模板设置之一标准曲线的绘制以及样品浓度的自动计算. The SpectraMax M5e possesses five preferred modes for multiple detection benefits in one platform: UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Time-Resolved Fluorescence (TRF), Fluorescence. NanoRacks added it to their line of commercial research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. 8 x 10-15. This document is provided to customers who have purchased Molecular Devices, Inc. For more information about the SpectraMax® M5e Multi-Mode Microplate Reader or other products from Molecular Devices, please visit our website (or send an email to. 3. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). After washing, the chromogenic substrate was added, and the OD was read using a SpectraMax M5e microplate spectrophotometer (Molecular Devices) at 450 nm. Introduction. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Description 1. Relative fluorescence units (RFU) were converted to units of NADPH concentration (μmol/L) using a standard curve of NADPH. Call 516-939-0888 to check current. The REF button has no valid meaning for. Italic and boldface type are used for emphasis. 6 x 10-15. Results summary for cAMP HiRange standard curve Figure 3. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. Endpoint, kinetic, spectrum. Fluorescence (FL). 3. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The following document is. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. If not, our scientific teamwill help you choose the instrument that best suits your needs and budget. SpectraMax® Aquamax. 1. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 91. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. We would like to show you a description here but the site won’t allow us. combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. 5. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. 0 out of 5 $ 24,695. Gemini EM. Introduction. With optimized reagents, option to operate the readers. About Conquer. SpectraMax M3, M4, M5, and M5e Multi-Mode Microplate Readers 0112-0115 F 9the assay on the SpectraMax Paradigm and M5e readers was evaluated by titration curves shown below. 00 - Original price $5,513. e. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAn example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. Example: [Drawer]. View the Molecular Devices website. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. SpectraMax Mini. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. The detector is a CCD array with 512 pixels in x and 512 pixels in y. SpectraMax M5e: 118. 0200-7027. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Explore PDF for more details. SpectraMax Gemini EM/XPS. SpectraMax L: 36. SpectraMax M2. Introduction. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. M3-M5e_manual. Fluorescent Polarization: Solid black plates, as above. SpectraMax Mini: 17. The mirror set. At the American Heritage School, located in Plantation, Florida, students used a SpectraMax® M5e to conduct scientific research during their first Annual Summer Science Program. Settings are for each instrument are shown in Table 1. SpectraMax M5e Multi-Mode Microplate Reader. Delivers single mode reader performance in one multimode reader package. SpectraMax Mini: 17. ₹ 1,80,000 Get Latest Price. cAMP HiRange 0. SpectraMax® iD5. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. -- JULY 19, 2016Cables. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. 97 cAMP Detection AssayMolecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Was the failure only of the Detection Estimate (Mag3) Test for the M3/M4/M5/M5e/Flex3? The M3/M4/M5/M5e/Flex3 multi-mode readers have a higher instrument noise level floor as compared to the dedicated luminometers. FP assay do not perform well with protein:protein interactions. UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range. Thermo Lab Systems Multiskan Ascent 354 $1,400. NanoRacks LLC officials have chosen Molecular Devices’ SpectraMax M5e Multi-Mode Microplate Reader for use in NASA's International Space Station. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SpectraMax DuoLuc Reporter Assay (SMP 6. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Protein melting curves were recorded using CFX96 thermocycler (Bio-Rad). • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. SpectraMax Mini: 17. Devices SpectraMax M5e (Ex/Em 380/650nm) • SoftMaxPro software allows easy data analysis Real-time data analysis SoftMaxPro software allows easy viewing of oxygen consumption profiles in all wells (Fig. C. the SpectraMax M5e, one of Molecular Devices most reliable and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. This document is provided to customers who have purchased Molecular Devices, Inc. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator the assay on the SpectraMax Paradigm and M5e readers was evaluated by titration curves shown below. The microplate reader made it possible for the students to perform higher level research using GFP-labeled C. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available) ATG for AKTA Explorer, Purifier and FPLC PMs, Repairs and Service Contracts. Table of Contents. The optical systemsProducts : SpectraMax M5/M5e reader Can BRET be read on the SpectraMax M5? BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. Cell culture U937 cells were cultured in RPMI-1640 medium supplemented with 10% FBS, 100ScanLater Western Blot Detection Cartridge. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromMolecular Devices SpectraMax™ M5e Microplate Reader w/ Warranty (Svc Contract Available) $34,000. 00 USD. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 现有酶标仪的客户基本都是需要进行数据分析，可能习惯使用Excel、Prism等软件，对我们SoftMax Pro的数据分析功能不了解，事实上大家可使用SoftMax Pro对数据进行完整处理并不需要再导入第三方. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。SpectraMax ® ® M5e readers must be appropriately configured for HTRF ® readout by setting up the measurement conditions in the SoftMax Pro software. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Table of Contents. Testing we performed: The Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader passed all tests and was found to be fully functional: Power: PASSED Initialization: PASSED Display/Buttons: PASSEDThe Challenge. Multimode Reader Comparison Chart.